Effects of recombinant human growth hormone (rhGH) administration on body composition and cardiovascular risk factors in obese adolescent girls

Background: Obesity is associated with a relative deficiency of growth hormone, which is predictive of greater visceral fat and markers of cardiovascular risk. The study’s purpose was to use recombinant human growth hormone (rhGH) as a physiologic probe to assess the effects of reversing obesity-related GH deficiency on body composition, cardiovascular risk markers, and insulin resistance. Methods: 22 obese girls 13–21 years old were followed for a randomized 6-month trial of rhGH vs. placebo/no treatment. At baseline and 6-months, DXA was performed for body composition, MRI to measure visceral, subcutaneous and total adipose tissue (VAT, SAT and TAT), and fasting blood drawn for IGF-1, inflammatory cardiovascular risk markers [soluble intercellular adhesion molecule (sICAM), high sensitivity CRP], lipids and HbA1C. An oral glucose tolerance test (OGTT) was performed. Twelve girls completed the 6-month visit. Baseline and mean 6-month change were compared between the groups using the Student t-test and the relationship between variables was determined through multiple regression analysis. Results: After 6-months, the rhGH group maintained IGF-1 levels, and had decreases in total cholesterol (p = 0.03), sICAM-1 (p = 0.04) and HbA1C (p = 0.03) compared to placebo/no treatment. The rhGH group trended towards greater decreases in LDL and 2-hour OGTT glucose. Glucose tolerance did not worsen with rhGH administration. Conclusions: Administering rhGH in small doses is able to stabilize IGF-1 levels in obesity. We have also shown that rhGH administration leads to an improvement in some markers of cardiovacular risk with without adversely affecting glucose tolerance. Trial registration Clinical Trial Registration Number: NCT01169103

Mapping contacts between regulatory domains of skeletal muscle TnC and Tnl by analyses of a single-chain chimeras.

The troponin (Tn) complex is formed by TnC, TnI and TnT and is responsible for the calcium-dependent inhibition of muscle contraction. TnC and TnI interact in an antiparallel fashion in which the N domain of TnC binds in a calcium-dependent manner to the C domain of TnI, releasing the inhibitory effect of the latter on the actomyosin interaction. While the crystal structure of the core cardiac muscle troponin complex has been determined, very little high resolution information is available regarding the skeletal muscle TnITnC complex. With the aim of obtaining structural information regarding specific contacts between skeletal muscle TnC and TnI regulatory domains, we have constructed two recombinant chimeric proteins composed of the residues 191 of TnC linked to residues 98182 or 98147 of TnI. The polypeptides were capable of binding to the thin filament in a calcium-dependent manner and to regulate the ATPase reaction of actomyosin. Small angle X-ray scattering results showed that these chimeras fold into compact structures in which the inhibitory plus the C domain of TnI, with the exception of residues 148182, were in close contact with the N-terminal domain of TnC. CD and fluorescence analysis were consistent with the view that the last residues of TnI (148182) are not well folded in the complex. MS analysis of fragments produced by limited trypsinolysis showed that the whole TnC N domain was resistant to proteolysis, both in the presence and in the absence of calcium. On the other hand the TnI inhibitory and C-terminal domains were completely digested by trypsin in the absence of calcium while the addition of calcium results in the protection of only residues 114137

Effects of phase separation on the magnetization, x-ray diffraction, and Raman scattering of (La1-yNdy)(1-x)CaxMnO3 (y=0,0.5,1.0; x=1/3)

Dc-magnetization, x-ray diffraction, and Raman-scattering studies in polycrystalline (La1-yNdy)(t-x)CaxMnO3 (y = 0.0,0.5,1.0, and x = 1/3) samples are presented. The samples with y = 0.5 and 1.0 show complex magnetic states at low temperatures, with a ferromagnetic coupling strength that weal;ens with increasing y. X-ray measurements show a single crystallographic phase at all temperatures for y = 0.5, with lattice parameter anomalies at temperatures related to electronic and magnetic transitions. The presence of high-frequency vibrational modes in Raman-scattering measurements indicates the existence of charge- and orbital-ordered domains for y=0.5 and 1.0, which are closely related to the antiferromagnetic component identified by the magnetization experiments. The close relationship between results obtained by magnetic, structural, and optical probes is discussed.63

Effects Of Phase Separation On The Magnetization, X-ray Diffraction, And Raman Scattering Of (la1-yndy) 1-xcaxmno3 (y = 0,0.5,1.0;x = 1/3)

Dc-magnetization, x-ray diffraction, and Raman-scattering studies in polycrystalline (La1-yNdy)1-xCaxMnO3 (y = 0.0,0.5,1.0, and x = 1/3) samples are presented. The samples with y = 0.5 and 1.0 show complex magnetic states at low temperatures, with a ferromagnetic coupling strength that weakens with increasing y. X-ray measurements show a single crystallographic phase at all temperatures for y=0.5, with lattice parameter anomalies at temperatures related to electronic and magnetic transitions. The presence of high-frequency vibrational modes in Raman-scattering measurements indicates the existence of charge- and orbital-ordered domains for y = 0.5 and 1.0, which are closely related to the antiferromagnetic component identified by the magnetization experiments. The close relationship between results obtained by magnetic, structural, and optical probes is discussed.636644041644046Wollan, E.O., Koehler, W.C., (1955) Phys. Rev., 100, p. 545Goodenough, J.B., (1955) Phys. Rev., 100, p. 564Chen, C.H., Cheong, S.-W., (1996) Phys. Rev. Lett., 76, p. 4042Radaelli, P.G., Cox, D.E., Marezio, M., Cheong, S.-W., Schiffer, P.E., Ramirez, A.P., (1995) Phys. Rev. Lett., 75, p. 4488Hwang, H.Y., Cheong, S.-W., Radaelli, P.G., Marezio, M., Batlogg, B., (1995) Phys. Rev. Lett., 75, p. 914Coey, J.M.D., Viret, M., Ranno, L., Ounadjela, K., (1995) Phys. Rev. Lett., 75, p. 3910Uehara, M., Mori, S., Chen, C.H., Cheong, S.-W., (1999) Nature (London), 399, p. 560Moreo, A., Yunoke, S., Dagotto, E., (1999) Science, 283, p. 2034. , and references thereinArchibald, W., Zhou, J.-S., Goodenough, J.B., (1996) Phys. Rev. B, 53, p. 14445Zhou, J.-S., Archibald, W., Goodenough, J.B., (1996) Nature (London), 381, p. 770Rao, G.H., Sun, J.R., Liang, J.K., Zhou, W.Y., Cheng, X.R., (1996) Appl. Phys. Lett., 69, p. 424Rao, G.H., Sun, J.R., Liang, J.K., Zhou, W.Y., (1997) Phys. Rev. B, 55, p. 3742Zhou, J.-S., Goodenough, J.B., (1998) Phys. Rev. Lett., 80, p. 2665Ibarra, M.R., Zhao, G.-M., De Teresa, J.M., García-Landa, B., Arnold, Z., Marquina, C., Algarabel, P.A., Ritter, C., (1998) Phys. Rev. B, 57, p. 7446Baszynski, J., Kovac, J., Kowalczyk, A., (1999) J. Magn. Magn. Mater., 195, p. 93Moritomo, Y., (1999) Phys. Rev. B, 60, p. 10374Young, R.A., Sakthivel, A., Moss, T.S., Paiva-Santos, C.O., (1995) J. Appl. Crystallogr., 28, p. 366Radaelli, P.G., Cox, D.E., Marezio, M., Cheong, S.-W., (1997) Phys. Rev. B, 55, p. 3015Huang, Q., Santoro, A., Lynn, J.W., Erwin, R.W., Borches, J.A., Peng, J.L., Ghosh, K., Greene, R.L., (1998) Phys. Rev. B, 58, p. 2684Irwin, J.C., Chrzanowski, J., Franck, J.P., (1999) Phys. Rev. B, 59, p. 9362Abrashev, M.V., Ivanov, V.G., Iliev, M.N., Chakalov, R.A., Chakalova, R.I., Thomsen, C., (1999) Phys. Status Solidi B, 215, p. 631Liarokapis, E., Leventouri, Th., Lampakis, D., Palles, D., Neumeier, J.J., Goodwin, D.H., (1999) Phys. Rev. B, 60, p. 12758Granado, E., Moreno, N.O., García, A., Sanjurjo, J.A., Rettori, C., Torriani, I., Oseroff, S.B., Tokura, Y., (1998) Phys. Rev. B, 58, p. 11435Yoon, S., Liu, H.L., Schollerer, G., Cooper, S.L., Han, P.D., Payne, D.A., Cheong, S.-W., Fisk, Z., (1998) Phys. Rev. B, 58, p. 2795Liu, H.L., Yoon, S., Cooper, S.L., Cheong, S.-W., Han, P.D., Payne, D.A., (1998) Phys. Rev. B, 58, pp. R10115Liu, K., Wu, X.W., Ahn, K.H., Sulchek, T., Chien, C.L., Xiao, J.Q., (1996) Phys. Rev. B, 54, p. 3007Granado, E., Pagliuso, P.G., Sanjurjo, J.A., Rettori, C., Oseroff, S.B., Causa, M.T., Butera, A., Schultz, S., (1998) Non-Crystalline and Nanoscale Materials, pp. 105-115. , edited by J. Rivas and M.A. López-Quintela (World Scientific, Singapore)Granado, E., Sanjurjo, J.A., Rettori, C., Prado, F., Sánchez, R.D., Caneiro, A., Oseroff, S.B., (2000) Phys. Status Solidi B, 220, p. 609Dediu, V., Ferdeghini, C., Matacotta, F.C., Nozar, P., Ruani, G., (2000) Phys. Rev. Lett., 84, p. 4489Adams, C.P., Lynn, J.W., Mukovskii, Y.M., Arsenov, A.A., Shulyatev, D.A., (2000) Phys. Rev. Lett., 85, p. 3954Dai, P., Fernandez-Baca, J.A., Wakabayashi, N., Plummer, E.W., Tomioka, Y., Tokura, Y., (2000) Phys. Rev. Lett., 85, p. 255

Design of ideal vibrational signals for stinkbug male attraction through vibrotaxis experiments

Many groups of insects utilize substrate-borne vibrations for intraspecific communication. This characteristic makes them a suitable model for exploring the vibrations as a tool for pest control in alternative to chemicals. The detailed knowledge of the species communication is a prerequisite to select the best signals to use. In this sense, this study aimed at exploring the use of substrate-borne vibrations for pest control of the brown marmorated stink bug (BMSB), Halyomorpha halys Stål (Heteroptera: Pentatomidae). To this purpose, in a first set of experiments, we identified the spectral and temporal characteristics that best elicit male responsiveness. Bioassays were conducted with artificial signals that mimicked the natural female calling signal. In a second part, we used the acquired knowledge to synthesize new signals endowed with different degrees of attractiveness in single and two choice bioassays using a wooden custom-made T stand

Assessment of abdominal fat compartments using DXA in premenopausal women from anorexia nervosa to morbid obesity

Objective: The purpose of this study was to test a newly developed DXA method for abdominal fat depot quantification in subjects with AN, normal weight, and obesity using CT as a gold standard. Design and Methods 135 premenopausal women (overweight/obese: n=89, normal-weight: n=27, AN: n=19); abdominal visceral adipose tissue (VAT), subcutaneous adipose tissue (SAT), and total adipose tissue (TAT) areas determined on CT and DXA. Results: There were strong correlations between DXA and CT measurements of abdominal fat compartments in all groups with the strongest correlation coefficients in the normal-weight and overweight/obese groups. Correlations of DXA and CT VAT measurements were strongest in the obese group and weakest in the AN group. DXA abdominal fat depots were higher in all groups compared to CT, with the largest % mean difference in the AN group and smallest in the obese group. Conclusions: A new DXA technique is able to assess abdominal fat compartments including VAT in premenopausal women across a large weight spectrum However, DXA measurements of abdominal fat were higher than CT, and this percent bias was most pronounced in the AN subjects and decreased with increasing weight, suggesting that this technique may be more useful in obese individuals

The genome of Bifidobacterium pseudocatenulatum IPLA 36007, a human intestinal strain with isoflavone-activation activity

Background: Bifidobacterium species, including Bifidobacterium pseudocatenulatum, are among the dominant microbial populations of the human gastrointestinal tract. They are also major components of many commercial probiotic products. Resident and transient bifidobacteria are thought to have several beneficial health effects. However, our knowledge of how these bacteria interact and communicate with host cells remains poor. This knowledge is essential for scientific support of their purported health benefits and their rational inclusion in functional foods. Results: This work describes the draft genome sequence of Bifidobacterium pseudocatenulatum IPLA 36007, a strain isolated as dominant from the feces of a healthy human. Besides several properties of probiosis, IPLA 36007 exhibited the capability of releasing aglycones from soy isoflavone glycosides. The genome contains 1,851 predicted genes, including 54 genes for tRNAs and fie copies of unique 16S, 23S and 5S rRNA genes. As key attributes of the IPLA 36007 genome we can mention the presence of a lysogenic phage, a cluster encoding type IV fimbriae, and a locus encoding a clustered, regularly interspaced, short, palindromic repeat (CRISPR)-Cas system. Four open reading frames (orfs) encoding \u3b2-glucosidases belonging to the glycosyl hydrolase family 3, which may act on isoflavone glycosides, were encountered. Additionally, one gene was found to code for a glycosyl hydrolase of family 1 that might also have \u3b2-glucosidase activity. Conclusion: The availability of the B. pseudocatenulatum IPLA 36007 genome should allow the enzyme system involved in the release of soy isoflavone aglycones from isoflavone glycosides, and the molecular mechanisms underlying the strain's probiotic properties, to be more easily understood

Altered miRNA processing disrupts brown/white adipocyte determination and associates with lipodystrophy

miRNAs are important regulators of biological processes in many tissues, including the differentiation and function of brown and white adipocytes. the endoribonuclease dicer is a major component of the miRNA-processing pathway, and in adipose tissue, levels of dicer have been shown to decrease with age, increase with caloric restriction, and influence stress resistance. Here, we demonstrated that mice with a fat-specific KO of dicer develop a form of lipodystrophy that is characterized by loss of intra-abdominal and subcutaneous white fat, severe insulin resistance, and enlargement and whitening of interscapular brown fat. Additionally, KO of dicer in cultured brown preadipocytes promoted a white adipocyte-like phenotype and reduced expression of several miRNAs. Brown preadipocyte whitening was partially reversed by expression of miR-365, a miRNA known to promote brown fat differentiation; however, introduction of other miRNAs, including miR-346 and miR-362, also contributed to reversal of the loss of the dicer phenotype. Interestingly, fat samples from patients with HIV-related lipodystrophy exhibited a substantial downregulation of dicer mRNA expression. Together, these findings indicate the importance of miRNA processing in white and brown adipose tissue determination and provide a potential link between this process and HIV-related lipodystrophy.NIHEllison FoundationJoslin Diabetes and Endocrinology Research Center coresMary K. Iacocca ProfessorshipAcademy of FinlandSigrid Juselius FoundationFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Harvard Univ, Sch Med, Joslin Diabet Ctr, Sect Integrat Physiol & Metab, Boston, MA 02115 USAUniversidade Federal de São Paulo, Dept Biophys, São Paulo, BrazilUniversidade Federal de São Paulo, Program Mol Biol, São Paulo, BrazilAstraZeneca R&D, Cardiovasc & Metab Dis iMed, Molndal, SwedenUniv Helsinki, Dept Med, Helsinki, FinlandMinerva Fdn, Inst Med Res, Helsinki, FinlandUniv Massachusetts, Sch Med, Program Mol Med, Worcester, MA USAMassachusetts Gen Hosp, Program Nutr Metab, Boston, MA 02114 USAHarvard Univ, Sch Med, Boston, MA USAUniversidade Federal de São Paulo, Dept Biophys, São Paulo, BrazilUniversidade Federal de São Paulo, Program Mol Biol, São Paulo, BrazilNIH: DK082659NIH: DK033201NIH: AI060354NIH: DK040561NIH: U24-DK093000Joslin Diabetes and Endocrinology Research Center cores: DK036836FAPESP: 2010/52557-0Web of Scienc